Wallach's Interpretation of Diagnostic Tests: Pathways to Arriving at a Clinical Diagnosis (1414 page)

   The RVP molecular assay tests for the major respiratory viruses commonly tested for surveillance and patient management. In addition, the RVP molecular assay is frequently used for the confirmation of negative results obtained by other methods, such as the rapid antigen assay, direct immunofluorescence, or EIA.

   Limitations

   The low limit of detection varies depending on the methods and viruses tested.

ROTAVIRUS FECAL ANTIGEN DETECTION

   Definition and Use

   This test is used for the diagnosis of enteric infections caused by Rotavirus.

   This test may be ordered for patients, usually young children, who present with a sudden onset of watery diarrhea, which is often preceded by vomiting.

   Unpreserved stool is submitted for testing.

   Rotavirus antigen in stool is detected by immunologic techniques using rotavirus-specific antibodies. LA and EIA formats are typically used. Sensitivity and specificity of commercial EIA assays are reported in the high 90% range.

   
Turnaround time:
<24 hours.

   Interpretation

   
Expected results:
Negative.

   Limitations

   LA tests have reported sensitivities less than the sensitivities of EIA assays. Assays may be less reliable in neonates.