Wallach's Interpretation of Diagnostic Tests: Pathways to Arriving at a Clinical Diagnosis (826 page)

   Low cross-reactivity with ecgonine methyl ester, norcocaine, ecgonine

   EIA for urine

   Target analyte: benzoylecgonine (metabolite)

   Cutoff concentration:

•   150 ng/mL

•   300 ng/mL

   Approximately 50–60% cross-reactivity with cocaine and cocaethylene

   Low cross-reactivity with EME, ecgonine

   Confirmation assays are commonly chromatography based regardless of the specimen.

   GC/MS

   Full scan mode for qualitative identification of cocaine, cocaethylene, and metabolites: limits of detection—20–50 ng/mL

   Selected ion-monitoring mode for quantitative analysis of serum, plasma for cocaine, cocaethylene, and metabolites: limits of quantitation—5–20 ng/mL

   LC/MSn (multiple MS)

   Multiple reaction–monitoring mode for qualitative or quantitative analysis of cocaine, cocaethylene, and metabolites: limits of detection/ quantitation—20–50 ng/mL

COLD AGGLUTININS

   Definition